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  5. Macherey-Nagel™ NucleoSpin™ Plasmid DNA

Macherey-Nagel™ NucleoSpin™ Plasmid DNA

Product Code. 12353358
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No. of Reactions:
10 Preps
250 Preps
50 Preps
Includes:
Lid
No Lid
Unit Size:
10 reactions
250 reactions
50 reactions
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Prepare small scale high-purity plasmid DNA rapidly. NucleoSpin™ Plasmid Columns come with or without a cap lid.

Features:

  • For rapid small scale preparation high-purity plasmid DNA
  • Fast procedure – 6 Minipreps in 25 min.
  • High capacity – up to 50μg of plasmid DNA
  • Chaotropic salt wash buffer – extra pure DNA

Applications:

  • High-copy plasmids from E. coli
  • Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
  • Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures
  • Supplementary protocol for M13 DNA available on request
  • Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

Specifications

Format Column
Isolation Technology Silica Membrane Technology
Sequencing Type DNA and RNA Sequencing
For Use With (Application) High-copy plasmids from E. coli
Includes Lid
No. of Reactions 250 Preps
Product Type Plasmid Columns
Quantity 2 mL
Sample Volume 2 to 10 mL
Size <50 kbp
Test Time 25 min.
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/2-pr
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