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  5. Macherey-Nagel™ NucleoSpin™ RNA, Mini kit for RNA purification

Macherey-Nagel™ NucleoSpin™ RNA, Mini kit for RNA purification

Product Code. 11922402
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No. of Reactions:
10
12
50
240
250
Format:
Mini Spin Column
QIAcube
Unit Size:
10 reactions
250 reactions
300mL
50 reactions
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Mini spin kit for isolation of RNA of highest integrity.

Features
  • DNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
  • NucleoSpin™ Filters (shredders) – efficient sample homogenization and reduction of viscosity
  • Up to 70 μg ready-to-use RNA
  • Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
Applications
  • RNA isolation from cultured cells, tissue (standard protocol)
  • RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
  • RNA from ≤ 100 μL biological fluids
  • RNA clean-up from reaction mixtures
  • RNA from samples stored in RNAlater™
  • RNA from saliva samples collected with Oragene™ RNA (Genotek)
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

Bacterial Content 70 μg
Detection Method Silica-membrane
Format Mini Spin Column
Isolation Technology Silica Membrane
DNase Yes
Final Product Type NucleoSpin™ RNA Kit
For Use With (Application) RNA isolation from cultured cells, tissue
No. of Reactions 50
No. of Samples 4
Product Type NucleoSpin™ RNA Kit
Quantity 1 Each
Sample Size <5 x 106 cultured cells, <30 mg human/animal tissue, <109 bacterial cells, <108 yeast cells
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Respiratory sensitiser Category 1
  • LONG-TERM AQUATIC HAZARD Chronic 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) / guanidine hydrochloride, ethanol
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