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Macherey-Nagel™ NucleoSpin™ RNA Virus 50 Preps
Description
NucleoSpin™ RNA Virus is designed for the isolation of viral nucleic acids from serum, plasma or any cell-free biological fluids, featuring a special type of membrane which has a high binding capacity for nucleic acids.
The procedure is rapid and easy. The lysate is incubated for 5 to 10min and applied to the NucleoSpin™ column. After two washing steps to remove contaminations the RNA can be eluted with water or low-salt buffer. The kit contains all necessary buffers, spin columns and detailed protocols. Generally, NucleoSpin™ RNA Virus is suited for the parallel purification of viral RNA and DNA (support protocol). For example, isolation of HBV DNA is performed with an additional proteinase K digest (enzyme not included in the kit). For isolation of viral DNA with highest sensitivities, the NucleoSpin™ Blood kit is recommended.
Kit components: NucleoSpin™ RNA Virus columns, 2mL collecting tubes, buffers, RNase-free water, carrier RNA.
• Silica membrane technology
• Typical recovery: >90%
• Sample material: 150μL serum, plasma, cell-free biological fluids
• Fragment size: 100bp to 30kb
• Elution volume: 50μL
• Binding capacity: 40μg
• Preparation time: 30min/4-6 preps
• Format: mini spin column
• Support protocol for parallel isolation of viral RNA and DNA (proteinase K required)
• Applicable for HCV, HBV, HAV, HIV, HSV, HPV, VZV, EBV, parvovirus B19, H5N1
The procedure is rapid and easy. The lysate is incubated for 5 to 10min and applied to the NucleoSpin™ column. After two washing steps to remove contaminations the RNA can be eluted with water or low-salt buffer. The kit contains all necessary buffers, spin columns and detailed protocols. Generally, NucleoSpin™ RNA Virus is suited for the parallel purification of viral RNA and DNA (support protocol). For example, isolation of HBV DNA is performed with an additional proteinase K digest (enzyme not included in the kit). For isolation of viral DNA with highest sensitivities, the NucleoSpin™ Blood kit is recommended.
Kit components: NucleoSpin™ RNA Virus columns, 2mL collecting tubes, buffers, RNase-free water, carrier RNA.
• Silica membrane technology
• Typical recovery: >90%
• Sample material: 150μL serum, plasma, cell-free biological fluids
• Fragment size: 100bp to 30kb
• Elution volume: 50μL
• Binding capacity: 40μg
• Preparation time: 30min/4-6 preps
• Format: mini spin column
• Support protocol for parallel isolation of viral RNA and DNA (proteinase K required)
• Applicable for HCV, HBV, HAV, HIV, HSV, HPV, VZV, EBV, parvovirus B19, H5N1
Specifications
Specifications
| Content And Storage | 18°C to 25°C |
| Format | Mini Prep |
| Sample Type | Cell-free Biological Fluids, Plasma, Serum |
| For Use With (Application) | Isolation of Viral Nucleic Acids |
| No. of Reactions | 50 Preps |
| Size | 100 bp−approx. 50 kbp |
| Target | Viral RNA and DNA |
| Volume (Metric) | < 150 L |
Safety and Handling
Signal Word
- Warning
- Flammable liquid Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P264-Wash with water thoroughly after handling.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
- MIXTURE LIST-Contains : guanidine hydrochloride, ethanol
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